Reactive sulfhydryl groups of the band 3 polypeptide from human erythrocyte membranes. Identification of the sulfhydryl groups involved in Cu2+-o-phenanthroline cross-linking.
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چکیده
منابع مشابه
Identification of the reactive sulfhydryl groups of S-adenosylmethionine synthetase.
S-Adenosylmethionine synthetase from Escherichia coli is rapidly inactivated by N-ethylmaleimide. In the presence of excess N-ethylmaleimide inactivation follows pseudo first-order kinetics, and loss of enzyme activity correlates with the incorporation of 2 eq of N-[ethyl-2-3H]maleimide/subunit. Preincubation of the enzyme with methionine and the ATP analog adenylylimidodiphosphate reduced the ...
متن کاملThe reactive sulfhydryl groups of microsomal cytochrome reductase.
The results of previous work have indicated (2, 3) that one sulfhydryl group of microsomal cytochrome reductase is essential for the interaction of nucleotides with this enzyme. These results, however, do not rule out the possibility that there are other reactive sulfhydryl groups on the native enzyme and that they are involved directly or indirectly in nucleotide-enzyme interactions. In the ex...
متن کاملSulfhydryl Groups in Proteins
Egg albumin in the native, unaltered state, does not give tests characteristic of sulfhydryl groups. When, however, this protein is treated in any one of several ways, such as by heat (8, 9, 17), ultraviolet irradiation (lo), shaking (lo), or by solution in urea or other amides (7), free sulfhydryl groups make their appearance. The amount of free -SH groups appearing in egg albumin through the ...
متن کاملLocalization of Erythrocyte Membrane Sulfhydryl Groups Essential for Glucose Transport
The reactions of three organic mercurial compounds, chlormerodrin, parachloromercuribenzoate (PCMB), and parachloromercuribenzenesulfonate (PCMBS) with intact red blood cells, hemolyzed red cells, hemoglobin solutions, and hemoglobin-free ghosts have been characterized. Both PCMB and PCMBS react with only 2 to 3 sulfhydryl groups per mole of hemoglobin in solution, whereas chlormerodrin reacts ...
متن کاملThe Reactions of the Sulfhydryl Groups of Human Hemoglobin &*
The rates at which the sulfhydryl groups on each chain of hemoglobin fi4 react with N-ethylmaleimide and iodoacetate have been measured. The sulfhydryl group at position 93 reacts with both alkylating agents at approximately the same rate as normal liganded hemoglobin. The sulfhydryl group at position 112, which is not available in normal hemoglobin, reacts in hemoglobin f14 and does so much mo...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1979
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)50531-7